POTENTIAL REGENERATIVE CELL AND ITS CULTURING METHOD

September 11, 2013
DR. RONGXIANG XU HAS RECENTLY BEEN GRANTED THE PATENT "POTENTIAL REGENERATIVE CELL AND ITS CULTURING METHOD" IN CHINA.

THE CLAIMS OF THE PATENT ARE TRANSLATED AS BELOW:

 

POTENTIAL REGENERATIVE CELL AND ITS CULTURING METHOD

 

ABSTRACT

The present invention relates to a type of cell - potential regenerative cell (PRC) capable of continuous proliferation, and generated mammal (including human) cells, tissues and tissue-organs by in vitro culture and replication of PRCs. The present invention also relates to the methods and cell growth regulators for culturing mammal (including human) PRCs, tissues, and tissue-organs.

CLAIMS(76)

1. A cell mixture including activated mammal (including human) PRCs capable of continuous proliferation, wherein said PRCs have proliferative potential of stem cells but exist in the tissue in the morphology of normal tissue cells. In vitro culture of said PRCs provides proliferative cells. Said cell mixture is obtained by the following methods: obtain mammal (including human) adult tissue cells and/or tissue; the said mammal (including human) adult tissue cells and/or tissue are cultured in a medium to activate the PRCs; activated PRCs are cultured to proliferate continuously and present stem cell characteristics; wherein a cell growth regulator is added in the medium, said cell growth regulator contains at least sterols dissolved in oil and baicalin at a concentration of 0.1%--2% by weight based on the total weight of the regulator.

 

2. The cell mixture according to claim 1, wherein said activated PRCs proliferate and differentiate in vitro into cells with same sources as compared to said PRCs.

 

3. The cell mixture according to claim 1, wherein said activated PRCs proliferate and differentiate in vitro into cells with different sources as compared to said PRCs.

 

4. The cell mixture according to claim 1, wherein said activated PRCs proliferate and differentiate in vitro into tissues with same sources as compared to said PRCs.

 

5. The cell mixture according to claim 1, wherein said activated PRCs proliferate and differentiate in vitro into tissues with different sources as compared to said PRCs.

 

6. The cell mixture according to claim 1, wherein said activated PRCs and the generated proliferative cells and tissues by the culture of said activated PRCs, can be used for in vitro biological models and transplantation therapy.

 

7. The cell mixture according to claim 1, wherein said activated PRCs can be used as biological models for scientific research and drug active ingredient screening, and for toxicology studies of pharmaceutical and food.

 

8. The cell mixture according to claim 1, wherein said activated PRCs can be used as biological models for cancer research

 

9. The cell mixture according to claim 1, wherein said activated PRCs can be used as biological models for research on all substances activating and adapting to the life.

 

10. A method for culturing the cell mixture including activated mammal (including human) PRCs, wherein the method comprises the steps of:

A, obtain mammal (including human) adult tissue cells and/or tissue;

B, the said mammal (including human) adult tissue cells and/or tissue are cultured in a medium to activate the PRCs;

C, activated PRCs are cultured to proliferate continuously and present stem cell characteristics;

wherein a cell growth regulator is added in the medium, said cell growth regulator contains at least sterols dissolved in oil and baicalin at a concentration of 0.1% - 2% by weight based on the total weight of the regulator.

 

11. The method according to claim 10, wherein said oil is animal oil or vegetable oil. The vegetable oil is selected from corn oil, peanut oil, cottonseed oil, safflower oil, tea tree oil, sesame oil, olive oil and soybean oil.

 

12. The method according to claim 10, wherein said sterols comprise of animal sterols or phytosterols. The animal sterols include cholesterol and all its natural or synthetic isomers and derivatives.

 

13. The method according to claim 12, wherein said sterols comprise of stigmasterol, β-sitosterol, ergosterol, γ-sitosterol, brassicasterol, α-spinasterol, 24-dehydrocholesterol, poriferasterol, daucosterol and all natural or synthetic isomers and derivatives, and combinations thereof.

 

14. The method according to claim 13, wherein said sterol is a combination of stigmasterol, β-sitosterol and brassicasterol.

 

15. The method according to claim 10, wherein the amount of the sterol in the cell growth regulator is 0.5% - 20% by weight based on the total weight of the regulator.

 

16. The method according to claim 15, wherein the amount of the sterol in the cell growth regulator is 1% - 10% by weight based on the total weight of the regulator.

 

17. The method according to claim 16, wherein the amount of the sterol in the cell growth regulator is 2% - 6% by weight based on the total weight of the regulator.

 

18. The method according to claim 10, wherein the cell growth regulator comprises also beeswax at a concentration of 1% - 20% by weight based on the total weight of the regulator.

 

19. The method according to claim 18, wherein the concentration of beeswax is 2% - 10% by weight based on the total weight of the regulator.

 

20. The method according to claim 19, wherein the concentration of beeswax is 3% - 6% by weight based on the total weight of the regulator.

 

21. The method according to claim 6, wherein the cell growth regulator comprises also propolis at a concentration of 0.1% - 30% by weight based on the total weight of the regulator.

 

22. The method according to claim 19, wherein the concentration of propolis is 1% - 20% by weight based on the total weight of the regulator.

 

23. The method according to claim 22, wherein the concentration of propolis is 5% - 10% by weight based on the total weight of the regulator.

 

24. The method according to claim 10, wherein the concentration of baicalin is 0.2% - 1% by weight based on the total weight of the regulator.

 

25. The method according to claim 10, wherein the concentration of baicalin is 0.5% - 1% by weight based on the total weight of the regulator.

 

26. The method according to claim 10, wherein the cell growth regulator comprises also obaculactone at a concentration of 0.1% - 2% by weight based on the total weight of the regulator. 

 

27. The method according to claim 26, wherein the concentration of obaculactone is 0.2% - 1% by weight based on the total weight of the regulator.

 

28. The method according to claim 27, wherein the concentration of obaculactone is 0.5% - 1% by weight based on the total weight of the regulator.

 

29. The method according to claim 10, wherein the cell growth regulator comprises also yellow berberine at a concentration of 0.001% - 2% by weight based on the total weight of the regulator. 

 

30. The method according to claim 29, wherein the concentration of yellow berberine is 0.002% - 0.5% by weight based on the total weight of the regulator.

 

31. The method according to claim 30, wherein the concentration of yellow berberine is 0.003% - 0.1% by weight based on the total weight of the regulator.

 

32. The method according to claim 10, wherein the cell growth regulator comprises also berberine at a concentration of 0.001% - 2% by weight based on the total weight of the regulator. 

 

33. The method according to claim 32, wherein the concentration of berberine is 0.002% - 0.5% by weight based on the total weight of the regulator.

 

34. The method according to claim 10, wherein the cell growth regulator comprises also narcotoline at a concentration of 0.001% - 2% by weight based on the total weight of the regulator. 

 

35. The method according to claim 34, wherein the concentration of narcotoline is 0.002% - 0.5% and 0.003% - 0.1% by weight based on the total weight of the regulator.

 

36. The method according to claim 10, wherein the cell growth regulator comprises also earthworm at a concentration of 0.001% - 2% by weight based on the total weight of the regulator. 

 

37. The method according to claim 36, wherein the concentration of earthworm is 0.002% - 0.5% by weight based on the total weight of the regulator.

 

38. The method according to claim 37, wherein the concentration of earthworm is 0.003% - 0.1% by weight based on the total weight of the regulator.

 

39. The method according to claim 10, wherein the cell growth regulator comprises also yellow berberine, berberine, and narcotoline.

 

40. The method according to claim 10, wherein the cell growth regulator comprises beeswax, propolis, baicalin, obaculactone, yellow berberine, berberine, narcotoline and earthworm.

 

41. The method according to claim 10, wherein the cell growth regulator is added in an amount of 10g-50g per 100ml medium.

 

42. The method according to claim 41, wherein the cell growth regulator is added in an amount of 20g-30g per 100ml medium.

 

43. The method according to claim 42, wherein the cell growth regulator is added in an amount of 20g per 100ml medium.

 

44. The use of a combination, which contains at least sterols dissolved in oil and baicalin at a concentration of 0.1% - 2% by weight based on the total weight of the regulator, in the preparation of the cell growth regulator.

 

45. The use according to claim 44, wherein said oil is animal oil or vegetable oil. The vegetable oil is selected from corn oil, peanut oil, cottonseed oil, safflower oil, tea tree oil, sesame oil, olive oil and soybean oil.

 

46. The use according to claim 44, wherein said sterols comprise of animal sterols or phytosterols. The animal sterols include cholesterol and all its natural or synthetic isomers and derivatives.

 

47. The use according to claim 44, wherein said sterols comprise of stigmasterol, β-sitosterol, ergosterol, γ-sitosterol, brassicasterol, α-spinasterol, 24-dehydrocholesterol, poriferasterol, daucosterol and all natural or synthetic isomers and derivatives, and combinations thereof.

 

48. The use according to claim 47, wherein said sterol is a combination of stigmasterol, β-sitosterol and brassicasterol.

 

49. The use according to claim 44, wherein the amount of the sterol in the cell growth regulator is 0.5% - 20% by weight based on the total weight of the regulator.

 

50. The use according to claim 45, wherein the amount of the sterol in the cell growth regulator is 1% - 10% by weight based on the total weight of the regulator.

 

51. The use according to claim 50, wherein the amount of the sterol in the cell growth regulator is 2% - 6% by weight based on the total weight of the regulator.

 

52. The use according to claim 44, wherein the cell growth regulator comprises also beeswax at a concentration of 1% - 20% by weight based on the total weight of the regulator.

 

53. The use according to claim 51, wherein the concentration of beeswax is 2% - 10% by weight based on the total weight of the regulator.

 

54. The use according to claim 53, wherein the concentration of beeswax is 3% - 6% by weight based on the total weight of the regulator.

 

55. The use according to claim 44, wherein the cell growth regulator comprises also propolis at a concentration of 0.1% - 30% by weight based on the total weight of the regulator.

 

56. The use according to claim 55, wherein the concentration of propolis is 1% - 20% by weight based on the total weight of the regulator.

 

57. The use according to claim 56, wherein the concentration of propolis is 5% - 10% by weight based on the total weight of the regulator.

 

58. The use according to claim 44, wherein the concentration of baicalin is 0.2% - 1% by weight based on the total weight of the regulator.

 

59. The use according to claim 58, wherein the concentration of baicalin is 0.5% - 1% by weight based on the total weight of the regulator.

 

60. The use according to claim 44, wherein the cell growth regulator comprises also obaculactone at a concentration of 0.1% - 2% by weight based on the total weight of the regulator. 

 

61. The use according to claim 60, wherein the concentration of obaculactone is 0.2% - 1% by weight based on the total weight of the regulator.

 

62. The use according to claim 60, wherein the concentration of obaculactone is 0.5% - 1% by weight based on the total weight of the regulator.

 

63. The use according to claim 44, wherein the cell growth regulator comprises also yellow berberine at a concentration of 0.001% - 2% by weight based on the total weight of the regulator. 

 

64. The use according to claim 63, wherein the concentration of yellow berberine is 0.002% - 0.5% by weight based on the total weight of the regulator.

 

65. The use according to claim 64, wherein the concentration of yellow berberine is 0.003% - 0.1% by weight based on the total weight of the regulator.

 

66. The use according to claim 44, wherein the cell growth regulator comprises also berberine at a concentration of 0.001% - 2% by weight based on the total weight of the regulator. 

 

67. The use according to claim 66, wherein the concentration of berberine is 0.002% - 0.5% by weight based on the total weight of the regulator.

 

68. The use according to claim 67, wherein the concentration of berberine is 0.003% - 0.1% by weight based on the total weight of the regulator.

 

69. The use according to claim 44, wherein the cell growth regulator comprises also narcotoline at a concentration of 0.001% - 2% by weight based on the total weight of the regulator. 

 

70. The use according to claim 44, wherein the cell growth regulator comprises also earthworm at a concentration of 0.001% - 2% by weight based on the total weight of the regulator. 

 

71. The use according to claim 70, wherein the concentration of earthworm is 0.002% - 0.5% by weight based on the total weight of the regulator.

 

72. The use according to claim 71, wherein the concentration of earthworm is 0.003% - 0.1% by weight based on the total weight of the regulator.

 

73. The use according to claim 44, wherein the cell growth regulator comprises any combination composed of substances selected from the group of beeswax, propolis, baicalin, obaculactone, yellow berberine, berberine, narcotoline and earthworm.

 

74. Any use according to claims 44-73, wherein it is used for in vitro culture and replication of cells and tissues of plant or mammal (including human).

 

75. Any use according to claims 44-73, wherein the culture medium contains said cell growth regulator.

 

76. The use according to claim 75, wherein said culture medium include: Eagle MEM culture medium and their derivatives; Ham's F-12 medium; RPMI 1640 medium; 199 medium; L15 medium ; Fischer medium; MB752 / 1 medium; CMRL1066 medium; McCoy5A medium; FBS; newborn calf serum; horse serum; rat tail collagen; hydrolyzed lactoprotein; chicken serum; rabbit serum; goat serum.