企业邮箱
Plan for Initiating Human Regenerative Life
2014年-04月-30日
来源：MEBO
Plan for Initiating Human Regenerative Life
Xu Rongxiang
Affiliation: Beijing Rongxiang Institute of Regenerative Medicine, Beijing，100020
【Key words】Human regenerative life; Initiation; Skin organ regeneration and rejuvenation; Skin column regeneration technique
【Article type】 Expert Lecture
Hello, everyone!
We have put our efforts in the R&D of Human Body Regenerative Restoration Science (HBRRS) for over 20 years. It is now reaching a new stage, an era of initiating human regenerative life. We are the dominating power in this field. However, speaking of how HBRRS is developed and its future direction, understanding the following would be a must. I may suggest that the new stage would be a milestone in the development of HBRRS. The title of today’s lecture is plan for initiating human regenerative life, in which I will summarize the issue of how to directly apply HBRRS.
History of Life Science and Initiation of Human Regenerative Life
1.1 Retrospection on History of Life Science
History of Life Science and Initiation of Human Regenerative Life
1.1 Retrospection on History of Life Science
I announced the current status of world life science last year at the Golden Biatec award ceremony. And, today, it is a need to give a retrospection once more. According to historical records, life science started from the medicine created and represented by Hippocrates of ancient Greece 2500 years ago, it was a scientific system studying the human life, including both traditional Chinese medicine and west medicine of that time, having developed for over 2500 years till now.
At the beginning of 19th century, some new features emerged in the development of life science, e.g. the application of microscopes and telescopes. The microscope expanded our horizon over tiny items and helped developing cytology. And, with the development of cytology, especially the alchemy, the chemistry of the human body was created during the process of decomposing the whole body into tiny parts. The composition of the human body was not determined until the genetic technology of peas was expanded to human genetics, which resulted in a scientific revolution - study of human cutting-edge science - starting from the nomination of RNA and DNA for human genetic materials at the beginning of last century (around 1907 ).
Research on the function and chemical structure of nucleic acids is within the range of genetics. From the beginning of the discovery of double helix structure of DNA by Watson and Crick under electron microscope, human beings turned arrogant ever since then and assumed that the code of human beings was unlocked; human could be built by using the double helix structure; and chronic diseases and cancer could be treated and eradicated through changing certain substance of this structure. The assumptions encouraged U.S. Presidents (the formers and the incumbent president) to develop this science at enormous cost for many years to come, dreaming to conquer refractory diseases. However, this dream was broken on 21st last March by Waston, the discoverer of DNA’s double helix structure, who declared it impossible to realize medical treatment through modification of gene. In 52 years, he tried all means to change the human genes but failed to cure any disease. Therefore, research on genes reached a deadlock after a century’s development. Although those of vested interest don’t want to deny this research, it will gradually slow down as it is the trend of science development.
Actually, it has already been disclosed that no disease could be treated by changing genes since 1980s. However, in order to get commercial benefit from the scientific system of huamn genetic study, human genome project (genome sequencing, valuable for physiological research but useless for disease treatment) was proposed and hyped up as if the sealed book of human life were found.
Soon after, this hype upsurge cooled down due to no breakthrough was achieved from the genome study. A replacing hype emerged instead. Embryology professor Thomson from Wisconsin of US announced the isolation of embryonic stem cells and proposed in vitro culture of organs by mimic of human embryonic development, which was awarded as one of the annual ten scientific technologies by Science in 1998. His research, though catching much attention, is actually embryonic stem cell line study, rather than embryonic stem cell research. Professional people all know what cell line means. Director Wang Yunping made many research on variable cell lines and cancer cells, his study together with many other scientists’ research, verified that once stem cell turn to stem cell lines (staying at the stage not able to develop into tissues), they lose their original property and transform into cancer cell like cells, while the original mission of stem cell is to form tissues. The hypers used the technology of stem cell line, but they intentionally ignored the word “line”and directly took it as stem cell. Either Nobel prize winners or other award winners, they did not mention “line”in the study of embryonic stem cell line, which misled others to naturally think of eggs, semen and embryo. They knew such research could not last long, so, they terminated it in four years by finding a reason of ethical violation in western society, In fact, the termination of this study is not due to ethics, it is a false topic from the very beginning and can not make any achievement. Therefore, we must understand it scientifically, it is embryonic stem cell line, not the concept of embryonic stem cell.
During the 4-year period of their hype, in 2002, we announced our regenerative science based on the achievement of converting somatic cells into pluripotent stem cells and regenerating tissue organs, shocking the life science community around the world. We are the only one of vested interest in this new field of science. No one can follow us even if they want to, because of what an expert of Chinese Academy of Science has asid “although their direction is wrong, they can not follow you, otherwise they can not get research fund.”
Soon after our announcement of the regenerative science, I was invited to give a special lecture in Chinese Academy of Science. At that time, my research was condemned by Prof. Zou Chenglu. On that day, Prof. Zou happened to give a lecture too upstairs. Someone came to my lecture saying “there are two presentations today, Zou Chenglu upstairs and Xu Rongxiang downstairs, Xu’s better”. It was CCTV Science and Technology channel recording my lecture and many Academicians came to my lecture after short stay upstairs We respect Prof. Zou as he is the pioneer biochemist in China which we had known when we announced the regenerative science. His denial of our science with violent words is somewhat a good thing to me, maybe he had the idea of my contribution when I was still unclear about what I had contributed.
Of course, our scientific research is quite different from theirs. The reason he strongly disagreed with us is that he was excluded from the Academician committee organized by Department of Science and Technology for reviewing my scientific research. Now we can say his denial is wrong. His violent words at that time were recorded in the development history of our science. I told reporters on the press conference held in Grand Hotel “remember the voice of denial, all the interference today will be jokes in the future.” We are not ambiguous in history, which is the real process of science development. At that time, a newspaper headlines commented on the conversion of somatic cells into stem cells, saying “absolutely impossible”.
We just did our research setting aside those interference, but unexpectedly, Japanese usurped our research achievement. Believing the pluripotency of somatic cells, Shinya Yamanaka of Kyoto University in Tokyo carried out study according to our idea. He manifested the pluripotency of somatic cells through gene transfer and published his first paper of this study in 2006 together with patent application. However, what used in his experiment is cell of chimeric mouse, which comes with property of mutation disposed to develop cancer. And then one year later, he and another American scientist replicated the same experiment and publicized their articles stating the pluripotency of somatic cells on Science and Cell respectively within the internal of one month. They named their pluripotent cell as Induced Pluripotent Stem Cell. However, it actually has nothing to do with real stem cell. He was just hyping systematically in 2006 and 2007 .
We just did our research setting aside those interference, but unexpectedly, Japanese usurped our research achievement. Believing the pluripotency of somatic cells, Shinya Yamanaka of Kyoto University in Tokyo carried out study according to our idea. He manifested the pluripotency of somatic cells through gene transfer and published his first paper of this study in 2006 together with patent application. However, what used in his experiment is cell of chimeric mouse, which comes with property of mutation disposed to develop cancer. And then one year later, he and another American scientist replicated the same experiment and publicized their articles stating the pluripotency of somatic cells on Science and Cell respectively within the internal of one month. They named their pluripotent cell as Induced Pluripotent Stem Cell. However, it actually has nothing to do with real stem cell. He was just hyping systematically in 2006 and 2007 .
By then, the investigator of clone sheep Dolly and Thomson of Wisconsin all declared to transfer to the research on inducing somatic cells into pluripotent stem cells. Thereafter, most scientists around the world joined this research field. But, who is on earth the founder of this research field? The year 2007 witnessed our announcements declaring our patent rights for the research on pluripotency of somatic cells.
In 2013, the international world of life science recognized this direction by accepting the pluripotency of somatic cells foe which Nobel Committee granted Yamanaka Nobel Prize in Phsyiology and Medicine. Meanwhile, we filed lawsuits against Nobel Committee to announce our ownership in this technology. Our strategy was first to disclose that their study was false because what they studied was not pluripotent stem cells, followed by letting the whole world become familiar with our scientific system in a natural manner and make impartial judgement on who is the inventor of this scientific system. We applied patents and made declarations in 2001, five years ahead of their publications. We demanded legal judgement on the inventor ( confirmed by the US patents and China patents, the fact they intentionally ignored), and claimed our ownership of regenerative science which can not be denied or usurped by anyone.
The iPS cell they hyped is not real stem cell. Pluripotency rather then stem cell has always been the focus of their hype. However, the property of cell’s pluripotency is our research achievement. In basic research, we have demonstrated the pluripotency of somaatic cells; In clinical application, we have accomplished regeneration of tissues and organs. Meanwhile, they have not yet achieved any real pluripotemt stem cell.
By the contents retrospected above, we should be clear that we are the only one standing out on the stage of human regenerative science. Those pretending to be us with pseudoscience absolutely have no courage to stand on this stage. This is the current status and our retrospection of history.
Although debate and discuss are necessary in the development of science, cheating is not allowed absolutely. We have confirmed this scientific system through solid research and will not let the fakers destroy it with their pseudoscience. We have to clean all the interference while making development, which is our general status of current development.
Today, after knowing about the history, we should get to know what we do next. Instead of staying at the stage of fundamental theoretical research and application, our science should move forward to a new stage of initiating human regenerative life (previously called world of regenerative life, i.e., the manifestation of human congenital regenerative life). In other words, our next goal is to initiate human regenerative life. In macroscopic view, this is our general target of political and economical development. .
Politically, we have determined to establish a scientific UN to guide the human beings to realize regenerative life; Economically, this organization will serve as economic lever in all countries to promote forming the world capital association; In science, we are the leader of regenerative life plan, and need to train qualified experts rapidly around all the world.
The start-up of the human regenerative life plan is the key point of unified international development of our science. We are the origin of this plan and must start first. Our strategy has been adjusted, we must lead the spreading and education of our science and technology and attract all the well-known scientists to join us.
1.2 Four Phases for Start-up Plan of Human Body Regeneration
The mission of Rongxiang Institute of Regenerative Medicine is to accomplish the four phases of regeneration of the human body. Today, I would like to elaborate on the details and means to complete the four phases for initiating human regenerative life.
The mission of Rongxiang Institute of Regenerative Medicine is to accomplish the four phases of regeneration of the human body. Today, I would like to elaborate on the details and means to complete the four phases for initiating human regenerative life.
We made some changes with regard to some terminologies about regeneration science. For example, we give up the term of regenerative health promotion. We used this term previously because we were at the first stage at that time, the exploration stage of human body regeneration, which focused on health promotion. But now, we have completed the first stage and got the principle rules and conclusions. Therefore, we stop calling health promotion and go directly to the stage of achieving human body regenerative life which we call regenerative life plan. Our research focuses on human body, with cells as the smallest life unit and the body as the largest. So, the regeneration of cells means the regeneration of the human body. This is an advance in concept, we’ll not use virtual terms any more. Nowadays, the term of “health promotion” has been abused, anything, even routine diet, can be taken as health promotion. The essence of our research is totally different from that. Our study objective is to initiate human regenerative life.
Before the invention of our science, we have only one life which means the development of our body, from birth to mature, aging and death. Now, we have found that somatic cells can be pluripotent, and regeneration of organs and tissues have been achieved. Therefore, we have established regenerative life science which system includes such concepts as somatic cells, stem cells and tissue organs that have been clearly indicated on the cover of Human Body Regeneration and Restoration Science. Then, how can somatic cells play a role of stem cells and develop into tissue organs and finally realize the regenerative life? The answer is the “plan for initiating hunam regenerative life” what I will elucidate today.
It will take 12 years to accomplish the plan for initiating hunam regenerative life. In the future, we shall inform all clients that they need to go through all four phases rather than only taking GIC and Elixir to accomplish this plan.
The first phase is the fundamental phase, featuring “41113” diet structure. This structure is based on the calculation of nutrition level in blood of ordinary people. The nutrition portfolio can be finally calculated for nutrition food for fundamental phase. Regenerative nutritional substance is created by the calculation based on the nutritional proportion for the human body and life conditions of healthy population. After this phase, the conditions of an ordinary person can be adjusted to be regenerative status. The first phase takes 4 to 5 years. We have accomplished the task of this phase and the future plan for initiating human regenerative life has emerged.
The second phase features spectrum portfolio characterized by the full-nutrition composition of human blood prepared according to the life standards required by red cells under regenerative status (blood nutritions and red cell’s nutritional conditions in the body of volunteer after finishing the first phase) . Based on the data collected, regenerative substances for gastrointestinal tract, all internal organs and skin can be made, and the full-nutrition diet (different from that in the first phase) for basic life can be designed. This year, we are going to start this phase in volunteers having finished the first phase. Our cell laboratory and analysis laboratory will run with full load.
The second phase features spectrum portfolio characterized by the full-nutrition composition of human blood prepared according to the life standards required by red cells under regenerative status (blood nutritions and red cell’s nutritional conditions in the body of volunteer after finishing the first phase) . Based on the data collected, regenerative substances for gastrointestinal tract, all internal organs and skin can be made, and the full-nutrition diet (different from that in the first phase) for basic life can be designed. This year, we are going to start this phase in volunteers having finished the first phase. Our cell laboratory and analysis laboratory will run with full load.
After being supplied with nutrition unique for red cells for 3 years, our body will be able to adapt to the comprehensive nutrients supply mechanism. The diet structure is also different from the first stage. Your diet is provided based on the full-nutrition spectrum required by red cells. All those qualified will be provided with their own unique daily diet plan which details the categories and volume of the daily food. You can imagine the status of the body having finished 3 years of red cell regeneration initiation. You will be younger both mentally and physically and your cells will be more vital, the effect of rejuvenation will be much more evident. That is also why the rats fed with regenerative substance did not age at the age double their average life span.
The third phase is the nutritional stage for stem cells (converted from human somatic cells). So far, no other scientists around the world can get stem cells by culture. We have already directly converted somatic cells into pluripotent stem cells. By collecting the tissue cells from the volunteers in this phase, we can culture these cells in lab and make nutrient solution for pluripotent stem cells (which absolutely has not been imaged by humans). The data about nutrient solutions and the data about full-nutrition analysis of the blood can be used to make the spectrum of regenerative substances in the third phase. The diet prepared based on this spectrum shall also be adopted for three years.
The fourth phase is the last step to start up the human regenerative life and to maintain a thriving status of your tissues. Based on the in vitro model of human bsomatic cells converting into stem cells and forming tissues, as well as the full nutrition data of the blood when entering the fourth phase, regenerative substance spectrum and the fundamental diet structure for the fourth phase can be prepared. This diet structure will serve as our life-long diet plan. In this phase, the plan for initiating human regenerative life will be completed and the human tissues will be totally in regenerative status.
The four phases of the plan for initiating regenerative life is the necessary scientific route of future human life science. It is a milestone in the development of life science. All above is not my imagination. Instead, it is a summary based on our experiment data and application rules. We have been analyzing and summarizing our experiences got over the past 4 to 5 years. Now, we determine to stop health promotion and really start the plan for initiating regenerative life on schedule. When human tissues are in totally regenerative status, most diseases, except trauma and infectious diseases, will be eradicated. By then, all of us will only die from aging rather than diseases. Additionally, our healthy life span will extend by at least one time, in accordance with the result of animal experiment. The 12 years for executing the plan for initiating regenerative life will lay a solid foundation for the human life. And, this is the application system of our science.
1.2.1 Indicators of Initiating Regenerative Life of Human Body in Different Phases
1.2.1 Indicators of Initiating Regenerative Life of Human Body in Different Phases
There are different indicators for each of the four phases of initiating human regenerative life. We have completed the research of the first phase and concluded with definite indicators. First of all, the apparent improvement in the aspect of spirit, qi, vigor and strength is noted from the perspective of function than before the regeneration. For instance, Xu, a volunteer with coronary heart disease, is now able to skip rope and climb the stairs as he was young, compared with the time before the initiation of regeneration three years ago when he would sustain from short of breath just walking few meters. His spirit, qi, vigor and strength are now keeping vigorous. In fact, the improvement was noted half a month after the initiation of the first phase. Where is the source of spirit, qi, vigor and strength? They are originated from the holistic exertion of function of human cells and tissue organs. Only if 80% of cells exert their functions, people is able to sense vigorous spirit, qi, vigor and strength. Therefore, we define spirit, qi, vigor and strength as the main indicators of the first phase. The secondary indicators of the first phase are the lab tests of diseased organs. In the first phase of initiating regenerative life of the human body, we observed that some diseases did not deteriorate while some indicator values of organ function turn better or even to the normal value, like parameters of coronary heart disease were totally turned normal three to five years after the initiation of regenerative life. The same thing has happened in the cases with GI problems and other organ diseases. The rejuvenation signs in skin and GI system have been found in some cases. There are no diagnostic techniques nowadays to determine if the other organs have been regenerated and rejuvenated. I believe the scientists in R&D of diagnostic medical instruments would try to invent those could diagnose the young status of cells when Human Body Regenerative Restoration Science would have been paid attentions by the scientific circle. Now we just can check the young status of reproductive cells. Above mentioned is the general objective of the first phase that can not be accomplished by modern medicine or natural health promotion.
The indictors of the second phase are still focused on some refractory organ diseases and rejuvenation status. The spirit, qi, vigor and strength are undoubtedly still the main indicators. Our aim in this phase is to keep maintaining vigorous spirit, qi, vigor and strength . For example, Xu who was mentioned in the first phase, will be in a better status in the second phase, which is certain.
The indicators of the third phase are totally focused on organ rejuvenation. In modern medicine, we can only detect the organ disease but not cell aging. Except cellular fibrosis, cellular hypofunction and hypometabolism are not detectable. However, when cellular fibrosis emerges, it is already late. There are no relevant means checking if a cell is young now, the situation is believed to be improved in a few years. By then, we will authorize some laboratories to specifically examine the young status of our volunteers’ organs.
The third phase is to achieve the transition from diseased status to the young status of organs with the activation of regenerative life. Subsequently, we are going to add the cellular vitality and the whole-spectrum nutrition of blood in the health report of our volunteers. What we provide is the indicators of normal nutrition and organ rejuvenation, different from modern hospitals’diagnostic indicators. Everyone can control his own health by then.
The third phase is to achieve the transition from diseased status to the young status of organs with the activation of regenerative life. Subsequently, we are going to add the cellular vitality and the whole-spectrum nutrition of blood in the health report of our volunteers. What we provide is the indicators of normal nutrition and organ rejuvenation, different from modern hospitals’diagnostic indicators. Everyone can control his own health by then.
The fourth phase is to maintain the relatively permanent healthy status of the body through the provision of personalized regenerative and fundamental nutrition.
Above is the overall route for the initiation of human regenerative life plan.
1.2.2 Scientific positioning of human regenerative life plan
We are going to carry out this start-up plan from the perspective of scientific R&D and will not do other research any more. Some relevant fundamental studies in this field all over the world might be useful in the future; as for applied studies, only ours has reached and accomplished practical application. We have completed the whole course of initiating regenerative life in animal experiment, as well as the first phase in humans, in which the overall rules of the following phases have been revealed. Once the scientific results and rules got from animal experiment and human test become law, we ought to achieve the human application in accordance with this law.
The work we are now doing is worthy to be a career for a lifetime because it is for the benefit of all human beings. We don’t care either being mocked or being awarded. We just do the real applied study and insist on the route and schedule. The US has included our organ regeneration science in its national policy, and the subsequent task is validation and full implementation. For now, several countries have intended to join this plan to accomplish the initiation of human regenerative life together. Our responsibility now is to hold the scientific technology within the scientific UN and to lead scientists all around the world to develop the science according to the plan for initiating regenerative life. Our primary task is to lay the foundations and to guide scientists from all countries to develop simultaneously. This is a developing way going beyond the control of a company or even a country.
2. Regenerative Renewal and Rejuvenation Technique of Skin Organs
To accomplish the plan of initiating human regenerative life requires us to achieve the initiation of regeneration of every single organ from outside. Today’s focus is regenerative renewal and rejuvenation of skin organ, i.e., plan for initiating skin organ’s regenerative life. According to years experience in the research on skin organ regeneration, we summarized three means to initiate skin organ’s regenerative life. .
To accomplish the plan of initiating human regenerative life requires us to achieve the initiation of regeneration of every single organ from outside. Today’s focus is regenerative renewal and rejuvenation of skin organ, i.e., plan for initiating skin organ’s regenerative life. According to years experience in the research on skin organ regeneration, we summarized three means to initiate skin organ’s regenerative life. .
2.1 Natural Skin Regeneration Technique
The first means is to regenerate skin in a natural way of smearing Regenerative Nutritional Substance (RNS) on skin by which skin will naturally absorb RNS and renew slowly via regeneration. Of course, oral administration of RNS has the same effect, but that is the means to initiate all organs’ regenerative lives in the body and will not be elucidated today. We call the means of external application of RNS “natural regeneration technique”. We are going to develop two dosage forms of products in future. One is a lipophilic liquid, and another is a water-soluble emulsion (containing less RNS but having higher absorbability). Based on personal skin quality and habit, one of the two products can be chosen to smear on the face before going to bed everyday and let it be absorbed naturally. In addition, we need to develop other ancillary products functioning to maintain a regenerative life environment. The ancillary products include skin care products and cosmetics. They also have regenerative function, e.g., primer and sunscreen cream. All the above products are capable of creating a physiological regenerative environment for the skin, which feature is used to create a series of same kind products. Among the two kinds of products mentioned above, one is a product containing pure RNS and the other is a product capable of creating a regenerative environment. They are the basic products to accomplish natural regeneration.
The first means is to regenerate skin in a natural way of smearing Regenerative Nutritional Substance (RNS) on skin by which skin will naturally absorb RNS and renew slowly via regeneration. Of course, oral administration of RNS has the same effect, but that is the means to initiate all organs’ regenerative lives in the body and will not be elucidated today. We call the means of external application of RNS “natural regeneration technique”. We are going to develop two dosage forms of products in future. One is a lipophilic liquid, and another is a water-soluble emulsion (containing less RNS but having higher absorbability). Based on personal skin quality and habit, one of the two products can be chosen to smear on the face before going to bed everyday and let it be absorbed naturally. In addition, we need to develop other ancillary products functioning to maintain a regenerative life environment. The ancillary products include skin care products and cosmetics. They also have regenerative function, e.g., primer and sunscreen cream. All the above products are capable of creating a physiological regenerative environment for the skin, which feature is used to create a series of same kind products. Among the two kinds of products mentioned above, one is a product containing pure RNS and the other is a product capable of creating a regenerative environment. They are the basic products to accomplish natural regeneration.
2.2 Strengthened Skin Regeneration Technique
After using the natural skin regeneration technique, if the slow speed of skin regeneration is not satisfying, strengthened skin regeneration technique can be used instead. Strengthened skin regeneration technique can help RNS permeate into the skin layers (epidermal and dermal layers) with a positive pressure of 100% Oxygen, it is the operation method we have been using all the time. As a result, the transported amount of RNS is much larger than the use of natural skin regeneration technique. The higher concentration of RNS should lead to a better result of skin regeneration and a faster speed of skin rejuvenation. Nevertheless, the series of skin care products mentioned above still need to be used during the interval period between the treatments in order to maintain the physiological regenerative environment and increase the daily absorption of RNS by the skin. As a matter of fact, this technique covers natural skin regeneration technique, the addition is the introduction of positive pressure gas to enhance its effect.
2.3 Skin Column Regeneration Technique
If the regenerating speed of strengthened skin regeneration technique is still not fast enough, our recently established skin column regeneration technique can be applied. The following slides will demonstrate the mechanisms of this technique. This new technique will be actively promoted all over the world. And I believe this technique will become the most popular, practical and authentic technique in skin regenerative renewal and rejuvenation in the near future. It helps the skin turn young and rejuvenated in a short time.
If the regenerating speed of strengthened skin regeneration technique is still not fast enough, our recently established skin column regeneration technique can be applied. The following slides will demonstrate the mechanisms of this technique. This new technique will be actively promoted all over the world. And I believe this technique will become the most popular, practical and authentic technique in skin regenerative renewal and rejuvenation in the near future. It helps the skin turn young and rejuvenated in a short time.
The mechanisms are as follows. The first step is to uphold the aged and flabby skin through a regenerated new skin column created by certain technique; the second step is to make the space between skin columns release a multitude of factors to promote new cell growth. The factors combined with the action of RNS, will increase greatly the overall amount of regenerative cells and regenerate and renew the skin gradually. This is the action mechanism of “skin column regeneration technique” in brief. Detailed mechanism is as below.
2.3.1 Action principle of skin column regenerative technique
The figure (Fig. 1) below is about the anatomy of skin, including epidermal and dermal layers. To rejuvenate the skin truly, it requires the rejuvenation of full-thickness skin rather than the rejuvenation of dermis or epidermis only.
2.3.1 Action principle of skin column regenerative technique
The figure (Fig. 1) below is about the anatomy of skin, including epidermal and dermal layers. To rejuvenate the skin truly, it requires the rejuvenation of full-thickness skin rather than the rejuvenation of dermis or epidermis only.
Fig. 1 Illustration of skin anatomy
The mechanisms of commonly used cosmetic techniques in epidermis rejuvenation in the market is to thin the epidermal layer. The reason why the cuticular layer of the aged skin becomes thicker is the slowed rate of metabolism of epidermis that caused by aged basal cells due to skin aging. For example, the vitality of epidermal basal cells in the 90-year-old is only 10% of the vitality when he is young. The vitality of human basal cells is inversely proportional to the age. But the skin column regenerative technique is the one that is able to increase the vitality of epidermal basal cells in the 90-year-old from 10% to 80%. In other words, it achieves the skin rejuvenation through the enhancement of basal cells vitality, the increased speed of epidermal renewal and the development of newly regenerated skin tissues. We have made the impressive achievement of converting epithelial cells in scar tissues into stem cells and regenerating new skin tissues. Rejuvenation of scarless normal aged skin is easier than scar skin.
The dermis has collagen fibers and skin appendages. Their vitality determines the fullness of skin tissues. The skin of a young girl always looks plump because the dermis of her skin is young, and vice versa. In an elder woman, the atrophy and apoptosis of the fibroblasts in her dermis layer result in the disorganized array of collagen fibers. Therefore, the way to rejuvenate our dermis is to reorganize the collagen fibers in dermis and to increase its amount and density. In addition, the skin appendages including hair follicles, vessels, nerve, sebaceous glands and sweat glands should also be rejuvenated, which is necessary for the complete rejuvenation of the skin. If skin appendages are not rejuvenated, the skin looks still aged and not vigorous. If skin appendages function normally, the skin looks vital, vigorous, and vivid. The vivid sense of skin relys on the exertion of normal function of arrectores pilorum.
The dermis has collagen fibers and skin appendages. Their vitality determines the fullness of skin tissues. The skin of a young girl always looks plump because the dermis of her skin is young, and vice versa. In an elder woman, the atrophy and apoptosis of the fibroblasts in her dermis layer result in the disorganized array of collagen fibers. Therefore, the way to rejuvenate our dermis is to reorganize the collagen fibers in dermis and to increase its amount and density. In addition, the skin appendages including hair follicles, vessels, nerve, sebaceous glands and sweat glands should also be rejuvenated, which is necessary for the complete rejuvenation of the skin. If skin appendages are not rejuvenated, the skin looks still aged and not vigorous. If skin appendages function normally, the skin looks vital, vigorous, and vivid. The vivid sense of skin relys on the exertion of normal function of arrectores pilorum.
The main evaluation indicators for the exertion of skin appendages function caused by RNS include: i. After using RNS, aged sebaceous glands with lowered or lost secretory and excretory function can be rejuvenated to recover normal secretory and excretory function; ii. The motion of aged hair follicles and arrectores pilorum is recovered; iii. The vascular network of papillary layer of dermis with the provision of good blood circulation becomes vigorous after the regeneration; iv. After regeneration of nerves, the skin recovers normal nervous reaction and looks vivid.
In summary, to make the aged skin rejuvenate is dependent on the renewal and rejuvenation of all tissues and cells in epidermis and dermis. The same results can never be realized by the current cosmetic therapies which just carry out some protective interventions on the skin that are totally unrelated with the metabolism of the cell. The above mentioned initiation of skin regenerative life is achieved through the exertion of pluripotency of skin PRC, the enhancement of present cells function to promote the renewal of skin tissues and cells in all layers and maintain the skin vitality.
In summary, to make the aged skin rejuvenate is dependent on the renewal and rejuvenation of all tissues and cells in epidermis and dermis. The same results can never be realized by the current cosmetic therapies which just carry out some protective interventions on the skin that are totally unrelated with the metabolism of the cell. The above mentioned initiation of skin regenerative life is achieved through the exertion of pluripotency of skin PRC, the enhancement of present cells function to promote the renewal of skin tissues and cells in all layers and maintain the skin vitality.
2.3.2 Difference between skin column regeneration technique and laser skin tightening
Skin column regeneration technique refers to the process of making micro pores down to the dermis (Fig. 2) through physical approaches like laser, acupuncture, electronic frequency needles and etc. Never mistake this technique for the laser skin tightening that is commonly used in plastic and cosmetic surgery.
Skin column regeneration technique refers to the process of making micro pores down to the dermis (Fig. 2) through physical approaches like laser, acupuncture, electronic frequency needles and etc. Never mistake this technique for the laser skin tightening that is commonly used in plastic and cosmetic surgery.
Fig.2 Creation of micro pores through physical approaches
Laser skin tightening means the creation of minor pores in the superficial layer of skin through the laser. The pores will be shrunken after the vaporization of skin tissues in the pores that results in the production of micro scarring tissue which tightens the surrounding skin tissues and decreases the skin size, letting the skin look younger. In other words, repeated using of laser skin tightening will leave much scar tissue inside the facial skin. In fact, the outcome of laser skin tightening is the shrunken status of skin caused by micro scars. Even though the skin looks smoother in appearance, the laser skin tightening destructs the skin histological structure actually and converts the physiologically normal skin into micro scarring skin, consequently resulting in stiff face or a face with obvious scars.
Totally different from laser skin tightening, aim of creating pores for skin column regenerative technique is to promote the absorption of RNS. The amount of RNS transported into the dermis is less when using strengthened skin regenerative technique. If the micro pores is down to the inside of dermis, relatively more RNS can be absorbed by dermis (Fig. 3), which is the secondary goal of the technique, the primary goal is to regenerate new skin columns in these micro cores.
Totally different from laser skin tightening, aim of creating pores for skin column regenerative technique is to promote the absorption of RNS. The amount of RNS transported into the dermis is less when using strengthened skin regenerative technique. If the micro pores is down to the inside of dermis, relatively more RNS can be absorbed by dermis (Fig. 3), which is the secondary goal of the technique, the primary goal is to regenerate new skin columns in these micro cores.
Fig. 3 RNS smeared on skin surface and introduced into skin by positive pressure of 100% oxygen.
Fig. 4-6 The newly regenerated skin column with epidermal and dermal tissues regeneration due to RNS
Tissue cells alongside the wall of pores in the dermal and epidermal layers will be activated and converted into stem cells (regenerative response, the same mechanism as burn management with MEBT/MEBO) after the introduction of RNS into skin. 24 hours after the introduction of RNS, the transformation of somatic cells into stem cells will be fully activated. 3 to 4 days after the introduction of RNS，new skin tissues will be formed. In other words, 3 days after the introduction of RNS, the space of pores will not be hollow again, instead they will be filled with newly developed epidermal and dermal tissues that we term as skin column. Nevertheless, how is the skin column regenerated? The vascularization is earlier than the growth of tissues. When RNS is accessible to the dermal tissues through the pores, the vessels in the dermis penetrate into the pores (Fig. 4), and then the cells and tissues start to develop. (The regeneration of micro-vessels is the concept of skin appendage regeneration.) Cells of collagen fibrous tissues (fibroblasts) in dermis will be transformed into precursor fibroblasts by the action of RNS (Fig. 5). Meanwhile, the cells in the basal layer of epidermis and along the wall of pores are also transformed into stem cells. (Fig. 6) This is the mechanism of regeneration as well as our patent---converting somatic cells into stem cells regenerating tissue organs. Three days after the introduction of RNS, when the vessels in the dermal layer extend into the pores with the new development of dermis and epidermis, the pores will be sealed so that there is no sense of pain while washing face (Fig. 7) . Therefore, no water is allowed on the affected skin within three days after the introduction of RNS because of the openness of the pores.
Fig. 7 Newly developed skin tissues filling the pores with the skin sealed by the regeneration of basement membrane and epidermis.
The skin column will protrude the facial skin early after formation. In other words, protruding and thickened facial skin is sensible at affected area three days after the introduction of RNS. At this time, feeling of rejuvenation emerges because of the upheld skin by the skin column (Fig. 7). One of our volunteers, after being applied skin column regeneration technique for 3 days, told us his face became round. This change cannot be achieved by using laser skin tightening, it is due to the thickened skin that is upheld by the newly regenerated skin columns.
After the sealing of the micro pores, the regeneration and renewal of surrounding tissues and cells is accelerated that results in the flatness of epidermal skin around the skin column (Fig. 8) due to the release of a multitude of growth factors - stimulating signals - produced by the new skin column. Just like the same result we obtained in the animal experiment of skin regeneration in scarring epidermis, that the number of stem cells in epidermal scar tissues is increased with the accelerated metabolism after the regeneration, meanwhile this regenerative response spreads to the surrounding skin tissues and leads their regeneration. The regenerative response in epidermis and dermis can be fully activated approximately in four weeks (Fig. 9) . It means the skin is completely renewed after four weeks of regeneration. But the optimal effect is obtained in the eighth week when the first course of treatment is completed and the skin regeneration and rejuvenation is fully achieved. (Fig. 10)
Fig. 8 The transformation of cells in dermis and epidermis into stem cells after the sealing of pores
Fig. 9 The rejuvenation of epidermis and dermis 4 weeks after the regeneration of skin columns.
Fig. 10 Skin regeneration and rejuvenation in all layers 8 weeks after the regeneration of skin columns
The above figures are prepared on the basis of observations on the histological sections through microscope and a patent application thereof is going to be filed. The reliability of these figures is definite.
2.3.3 Standing in the world and future development of Skin Column Regeneration Technology
We are the first organization having this technology in the world. Its mechanism is still in situ regeneration science and technology which is applied to the skin in a different way. It is applicable not only to normal aged skin and skin scar, but also to the abnormal skin with stretch marks, etc. The only change is the position and amount of the punched pores. No matter it is a normal skin, scar skin, or abnormal skin, regenerated skin columns are all physiological tissues. New skin columns are regenerated in scar and the scar will be gradually replaced and eliminated. As a simple method, skin column regeneration technique solves the problem of transformation of abnormal skin to normal, it is a revolutionary method.
This technology will likely change human appearance, while generating the greatest epoch-making economic benefits. Moreover, this is only the third method for implementing skin regenerative renewal and rejuvenation technique. The fourth, fifth and more methods are in tests. Techniques of natural regeneration, strengthened skin regeneration and skin column regeneration compose a complete set of technology to rejuvenate human skin. Our products include basic Regenerative Substance and Emulsified Regenerative Substance in nanometer scale for which complete cell regeneration experiments have been done. The absorption of Emulsified Regenerative Substance in the nanometer scale is 50 times more than the Basic Regenerative Substance. In the future, we may also develop spray.
What is the pure technology of skin organ regenerative renewal and rejuvenation? It is the portfolio of above mentioned three methods: natural regeneration method, strengthened regeneration method and skin column regeneration technique. There are no same techniques or even same term in the world. Any technique mentioning the term of in situ regeneration infringes upon our rights of patent, copyright and invention. However, we would not like to have a monopoly on this technology and we want to contribute it to benefit the mankind. We will take it to the world this year.
The natural regeneration method and strengthened regeneration method are non-invasive while skin column regeneration technique is a minimal invasive method. The purpose of the minimum invasive method is to create a physiological regenerative environment which can help the new skin column and new skin tissues grow in situ to uphold the surrounding tissues and to rejuvenate the skin. Skin column regeneration technique can be applied repeatedly after one course of 8 weeks. One of our volunteers has been treated for three courses and turned younger and younger. Why skin column regeneration technique can be applied repeatedly? It depends on the distance between micro pores. For the sake of safety, our designed distance is relatively big that the regenerated skin columns only cover 2% of the total facial skin after one course of treatment. Moreover, there is no scarring in the pores but regeneration of new skin tissues. Therefore it can be used repeatedly. In contrast, fractional laser treatment results in micro scars in all pores and consequently leads to a stiff face. Our skin regenerative renewal and rejuvenation technique results in pure physiological regeneration of tissues and organs, it is a transition of Burn Regenerative Medicine and Therapy to cosmetology, an undoubtedly scientific technology.
Initiation of skin organ regeneration is firstly introduced today. In the future, the regenerative renewal and rejuvenation of other organs will be introduced one by one, and the next will be heart. Why? Because heart is vital to the life a person. Once coronary heart disease is found in a person, the average 5-year mortality rate is as high as 50%, it is the first killer of human life. Another introduction will be for GI organ since GI is the most important organ for guarantee of energy supply and nutrition absorption of the human body. Only if GI organ is enhanced, the regenerative renewal and rejuvenation of other organs can be conducted. We are going to propose to the US Government a plan to reduce the coronary heart disease incidence in two years, to get rid of the hurt to humans by coronary heart disease, and to eliminate some currently hard to cure GI diseases.
Currently the imaging technology is developed, but we hope it can be better to be able to show blood capillary. In our standard, the amount of blood capillary is the index of heart quality. In addition, we need to see the overall blood supply of cardiac muscles because sufficient blood supply indicates a healthy heart.
So far, we have had thorough study on blood vessels. Recalling the study of blood vessels in burn wound on face and abdomen in 1996, it is clear that keeping smooth growth of blood vessels is necessary for the wound healing and tissue organ regeneration; once the growth of blood vessels is blocked, scar will form on the healed wound, and that is why we choose the exposed therapy instead of bandage therapy. When bandaged, the wound can heal but it is not physiological healing. When a seed sprouts, it pushes the soil cover on the top, this is a natural rule. But if the soil is watered, the cover can not be opened by the sprout. Similarly, if eschar covers the wound, the blood vessels can only grow peripherally leading to bad skin metabolism and disorderly growth of fibrocytes which ultimately forms a scar.
Blood vessels are the “steel bar” of physiological structure and play a role of framework. In the application of skin column regeneration technique, micro pores are made to let blood vessels enter, grow and connect to form a physiological framework followed by promoting the regeneration of nerves and fibroblasts to finally form new skin tissues closing the micro pores.
If the skin is hurt by a cut, plenty of substances will be exudated to close the wound which is a post-injury physiological self-repair response of the body. But, as for the regeneration of burn wound or skin columns, the wound or pores must not be blocked by exudates, otherwise scar will form.
When skin column regeneration technique is applied, after the preparation of micro pores by physical , method, regenerative substance is introduced into the pores, certain duration later, the content in pores must be extracted and introduce new regenerative substance again. Although the introduced is regenerative substance, the extracted is exudates. There is less early exudates in the pores made by Fractional laser; while there is much more exudates with puncture method. The reason is there are carbonized tissues in the pores made by fractional laser, it will take time for regenerative substance to soften and dissolve these carbonized tissues, during which period the regenerative substance need to be renewed every day to avoid the exuded cellulose being detained in micro pores and forming scar tissues,
When skin column regeneration technique is applied, after the preparation of micro pores by physical , method, regenerative substance is introduced into the pores, certain duration later, the content in pores must be extracted and introduce new regenerative substance again. Although the introduced is regenerative substance, the extracted is exudates. There is less early exudates in the pores made by Fractional laser; while there is much more exudates with puncture method. The reason is there are carbonized tissues in the pores made by fractional laser, it will take time for regenerative substance to soften and dissolve these carbonized tissues, during which period the regenerative substance need to be renewed every day to avoid the exuded cellulose being detained in micro pores and forming scar tissues,
3. Summary:
The above information is for the lecture today. Later on, I will present the initiation of organ regeneration for other organs one by one. So, everyone should be familiar with human body organ histology. We only acknowledge the organ histology and do not accept the organ pathology and medical therapeutics. Because so far in the world, not even one organ disease has been completely cured. We will complete the regenerative renewal and rejuvenation of every organ and provide the mankind a practical and reliable technique for well-beings.
Since 2013, we have attracted the world’s attention and our patents have been downloaded 160 Million times. They researched and failed to find any flaw in our patents, because our technology is true and repeatable On January 01 of lunar calendar of 2014, a woman Japanese scientist published the result of transformation of somatic cells into stem cells, however she copied the iPS cell concept of Shinya Yamanaka, and within only one month since her publication, many flaws were found and the study was denied by the scientist around the world due to the fake picture in her paper. However, somatic cell having pluripotency is a reality confirmed by us in many studies, like the renewal of skin which can be repeated on anybody.
We will keep moving forward and establish a new scientific route for human beings.
(Edited on the basis of audio records of the lecture held on March 14 2014)